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Gbx1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - Gbx1 Polyclonal Antibody AP54586
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gbx1) Polyclonal Antibody, Unconjugated (bs-11660R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • FC - Gbx1 Polyclonal Antibody AP54586
    Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
    Primary Antibody:Rabbit Anti-Gbx1 antibody(bs-11660R), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
    Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF, ICC
Primary Accession Q14549
Reactivity Rat, Pig, Chimpanzee, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 37629 Da
Additional Information
Gene ID 2636
Other Names Homeobox protein GBX-1, Gastrulation and brain-specific homeobox protein 1, GBX1
Dilution Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1 µg/Test,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name GBX1
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
Research Areas
Citations (0)
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$ 350.00
Cat# AP54586
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