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Gbx1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - Gbx1 Polyclonal Antibody AP54586
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gbx1) Polyclonal Antibody, Unconjugated (bs-11660R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • FC - Gbx1 Polyclonal Antibody AP54586
    Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
    Primary Antibody:Rabbit Anti-Gbx1 antibody(bs-11660R), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
    Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF, ICC, E
Primary Accession Q14549
Reactivity Rat, Pig, Chimpanzee, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 38 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human Gbx1
Epitope Specificity 201-300/363
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Nucleus.
SIMILARITY Contains 1 homeobox DNA-binding domain.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions The homeobox is DNA sequence that is approximately 180 base pairs long that is involved in all steps of the developmental regulation of animals, fungi and plants, from embryogenesis to cell differentiation. The homeobox encodes a protein domain of about 60 amino acids, called the homeodomain, that can bind to DNA. The gastrulation brain homeobox 1 (GBX1) gene, along with another homeobox gene (EN2), maps to chromosome 7q36, and is expressed in septal and pallidal areas and in the basal forebrain cholinergic system, an area implicated in Alzheimer’s disease. The GBX1 gene encodes the GBX1 protein, which localizes to the nucleus and contains 1 homeobox DNA-binding domain.
Additional Information
Gene ID 2636
Other Names Homeobox protein GBX-1, Gastrulation and brain-specific homeobox protein 1, GBX1
Dilution IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,Flow-Cyt=1 µg/Test,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name GBX1
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
Research Areas
Citations (0)
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$ 385.00
Cat# AP54586
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