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GLOD4 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - GLOD4 Polyclonal Antibody AP56189
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLOD4) Polyclonal Antibody, Unconjugated (bs-16250R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF, ICC, E
Primary Accession Q9HC38
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 35 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human GLOD4
Epitope Specificity 101-200/313
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Mitochondrion.
SIMILARITY Belongs to the glyoxalase I family.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions GLOD4 is a 313 amino acid protein belonging to the glyoxalase I family. GLOD4 interacts with NUDT9, a highly specific adenosine diphosphate ribose pyrophosphatase. Localized to mitochondrion, GLOD4 is expressed in heart, brain, liver, kidney, pancreas and placenta, but is not found in skeletal muscle or lung. Expression of GLOD4 is decreased in hepatocellular carcinoma samples in comparison to adjacent non-cancerous liver tissues from the same patients. Transfection of GLOD4 in hepatocellular carcinoma cells and overexpression has also been shown to inhibit cell growth. GLOD4 exists as three alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 17p13.3.
Additional Information
Gene ID 51031
Other Names Glyoxalase domain-containing protein 4, GLOD4, C17orf25
Target/Specificity Expressed in heart, brain, liver, kidney, pancreas and placenta. Not expressed in skeletal muscle and lung.
Dilution IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name GLOD4
Synonyms C17orf25
Cellular Location Mitochondrion.
Tissue Location Expressed in heart, brain, liver, kidney, pancreas and placenta. Not expressed in skeletal muscle and lung
Research Areas
Citations (0)
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$ 385.00
Cat# AP56189
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