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TRAF5 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - TRAF5 Polyclonal Antibody AP56319
    Paraformaldehyde-fixed, paraffin embedded (rat prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRAF5) Polyclonal Antibody, Unconjugated (bs-16563R) at 1:5000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
  • WB - TRAF5 Polyclonal Antibody AP56319
    Sample:
    Hela Cell (Human) Lysate at 30 ug
    Primary: Anti- TRAF5 (bs-16563R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 64 kD
    Observed band size: 64 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession O00463
Reactivity Rat, Pig
Host Rabbit
Clonality Polyclonal
Calculated MW 64406 Da
Additional Information
Gene ID 7188
Other Names TNF receptor-associated factor 5, RING finger protein 84, TRAF5, RNF84
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name TRAF5
Synonyms RNF84
Function Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF- kappa-B by EIF2AK2/PKR.
Cellular Location Cytoplasm. Cytoplasm, cytosol
Tissue Location Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood
Research Areas
Citations (0)
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$ 350.00
Cat# AP56319
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