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TRIM50 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | Q86XT4 |
| Other Accession | NP_835226.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 54728 Da |
| Antigen Region | 354-383 aa |
| Gene ID | 135892 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase TRIM50, 632-, Tripartite motif-containing protein 50, TRIM50, TRIM50A |
| Target/Specificity | This TRIM50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-383 amino acids from the C-terminal region of human TRIM50. |
| Dilution | FC~~1:10~50 IHC-P~~1:50~100 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TRIM50 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TRIM50 (HGNC:19017) |
|---|---|
| Synonyms | TRIM50A |
| Function | E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1 (PubMed:29604308). In turn, promotes starvation-induced autophagy activation. Also interacts with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction (PubMed:22792322). Also promotes NLRP3 inflammasome activation by directly inducing NLRP3 oligomerization independent of its E3 ligase function (By similarity). |
| Cellular Location | Cytoplasm Note=Localizes mainly into discrete cytoplasmic punctuate structures heterogeneous in size and shape containing polyubiquitinated proteins |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
TRIM50 belongs to the TRIM/RBCC family. It contains 1 B box-type zinc finger, 1 B30.2/SPRY domain and 1 RING-type zinc finger. TRIM50 is an E3 ubiquitin-protein ligase.
References
Micale, L., et al. Eur. J. Hum. Genet. 16(9):1038-1049(2008)
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