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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PPP3CC Polyclonal Antibody   

PPP3CC Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PPP3CC Polyclonal Antibody AP57737
    Sample: A549 (Human)Cell Lysate at 40 ug
    Primary: Anti-PPP3CC(bs-19973R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
    Predicted band size: 58kD
    Observed band size: 58kD
    detail
  • WB - PPP3CC Polyclonal Antibody AP57737
    Sample: Testis(Mouse)Lysate at 40 ug
    Primary: Anti-PPP3CC(bs-19973R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
    Predicted band size: 58kD
    Observed band size: 58kD
    detail
  • IHC-P - PPP3CC Polyclonal Antibody AP57737
    Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP3CC) Polyclonal Antibody, Unconjugated (bs-19973R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
    detail
  • WB - PPP3CC Polyclonal Antibody AP57737
    Sample:
    DU145(Human) Cell Lysate at 40 ug
    Primary: Anti-PPP3CC (bs-19973R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 58 kD
    Observed band size: 58 kD
    detail
  • WB - PPP3CC Polyclonal Antibody AP57737
    Sample:
    Hela(Human) Cell Lysate at 40 ug
    Primary: Anti-PPP3CC (bs-19973R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 58 kD
    Observed band size: 58 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession P48454
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 58129 Da
Additional Information
Gene ID 5533
Other Names Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform, 3.1.3.16, CAM-PRP catalytic subunit, Calcineurin, testis-specific catalytic subunit, Calmodulin-dependent calcineurin A subunit gamma isoform, PPP3CC, CALNA3, CNA3
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name PPP3CC
Synonyms CALNA3, CNA3
Function Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Dephosphorylates and activates transcription factor NFATC1. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32.
Cellular Location Mitochondrion {ECO:0000250|UniProtKB:P48455}. Note=Localizes in the mitochondria in a SPATA33-dependent manner {ECO:0000250|UniProtKB:P48455}
Tissue Location Testis..
Research Areas
Citations (0)
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$ 350.00
Cat# AP57737
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