PAM/Peptidylglycine 2 hydroxylase Polyclonal Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IHC-F, IF, ICC |
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Primary Accession | P19021 |
Reactivity | Rat, Pig, Dog, Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 108332 Da |
Gene ID | 5066 |
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Other Names | Peptidyl-glycine alpha-amidating monooxygenase, PAM, Peptidylglycine alpha-hydroxylating monooxygenase, PHM, 1.14.17.3, Peptidyl-alpha-hydroxyglycine alpha-amidating lyase, 4.3.2.5, Peptidylamidoglycolate lyase, PAL, PAM {ECO:0000303|PubMed:12699694, ECO:0000312|HGNC:HGNC:8596} |
Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
Format | 0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce |
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃. |
Name | PAM {ECO:0000303|PubMed:12699694, ECO:0000312|HGNC:HGNC:8596} |
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Function | Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides (PubMed:12699694). Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monooxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate (PubMed:12699694). The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc- dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate (PubMed:12699694). Similarly, catalyzes the two- step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (By similarity). |
Cellular Location | Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000250|UniProtKB:P10731}; Single-pass membrane protein {ECO:0000250|UniProtKB:P10731}. Note=Secretory granules {ECO:0000250|UniProtKB:P10731} [Isoform 2]: Membrane; Single-pass type I membrane protein [Isoform 4]: Secreted. Note=Secreted from secretory granules |
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