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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   IL22BP Polyclonal Antibody   

IL22BP Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - IL22BP Polyclonal Antibody AP58041
    Sample:
    testis(rat) Lysate at 40 ug
    Primary: Anti-IL22BP (bs-2625R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 25/28kD
    Observed band size: 35 kD
    detail
  • IHC-P - IL22BP Polyclonal Antibody AP58041
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL22BP) Polyclonal Antibody, Unconjugated (bs-2625R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • FC - IL22BP Polyclonal Antibody AP58041
    Blank control: mouse spleen sells(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
    Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1 µg in 100 µL1X PBS containing 0.5% BSA(green).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q969J5
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 30550 Da
Additional Information
Gene ID 116379
Other Names Interleukin-22 receptor subunit alpha-2, IL-22 receptor subunit alpha-2, IL-22R-alpha-2, IL-22RA2, Cytokine receptor class-II member 10, Cytokine receptor family 2 member 10, CRF2-10, Cytokine receptor family type 2, soluble 1, CRF2-S1, Interleukin-22-binding protein, IL-22BP, IL22BP, ZcytoR16, IL22RA2
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1 µg /test,IF=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name IL22RA2
Function Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.
Cellular Location Secreted.
Tissue Location Expressed in placenta, spleen, breast, skin and lung. Also detected in intestinal tract, testis, brain, heart and thymus. No expression found in prostate, bladder, kidney, ovary, muscle, bone marrow, liver and uterus. Isoform 1 is expressed only in placenta. Isoform 2 is expressed in placenta and breast and at lower level in spleen, skin, thymus and stomach
Research Areas
Citations (0)
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$ 350.00
Cat# AP58041
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