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C3a Receptor Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - C3a Receptor Polyclonal Antibody AP58066
    Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C3a Receptor) Polyclonal Antibody, Unconjugated (bs-2955R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • WB - C3a Receptor Polyclonal Antibody AP58066
    Sample:
    K562(Human) Cell Lysate at 30 ug
    293T(Human) Cell Lysate at 30 ug
    A549(Human) Cell Lysate at 30 ug
    Lung (Mouse) Lysate at 40 ug
    Primary: Anti-C3a Receptor (bs-2955R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 54 kD
    Observed band size: 64 kD
    detail
  • WB - C3a Receptor Polyclonal Antibody AP58066
    Sample: A549 Cell (Human) Lysate at 30 ug
    Primary: Anti-C3a Receptor (bs-2955R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 54kD
    Observed band size: 60kD
    detail
  • WB - C3a Receptor Polyclonal Antibody AP58066
    Sample: 293T Cell (Human) Lysate at 30 ug
    Primary: Anti-C3a Receptor (bs-2955R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 54kD
    Observed band size: 60kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q16581
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 53864 Da
Additional Information
Gene ID 719
Other Names C3a anaphylatoxin chemotactic receptor, C3AR, C3a-R, C3AR1, AZ3B, C3R1, HNFAG09
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name C3AR1
Synonyms AZ3B, C3R1, HNFAG09
Function Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues
Research Areas
Citations (0)
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$ 350.00
Cat# AP58066
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