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Squalene Epoxidase Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Squalene Epoxidase Polyclonal Antibody AP58135
    Sample:
    NIH/3T3(Mouse) Cell Lysate at 30 ug
    A549(Human) Cell Lysate at 30 ug
    Primary: Anti-Squalene Epoxidase (bs-4218R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 64 kD
    Observed band size: 64 kD
    detail
  • IHC-P - Squalene Epoxidase Polyclonal Antibody AP58135
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-Squalene Epoxidase Polyclonal Antibody, Unconjugated(bs-4218R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q14534
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 63923 Da
Additional Information
Gene ID 6713
Other Names Squalene monooxygenase, 1.14.14.17, Squalene epoxidase, SE, SQLE, ERG1
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name SQLE
Synonyms ERG1
Function Catalyzes the stereospecific oxidation of squalene to (S)- 2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.
Cellular Location Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein
Tissue Location Detected in liver (at protein level).
Research Areas
Citations (0)
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$ 350.00
Cat# AP58135
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