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DBF4B Polyclonal Antibody
Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | Q8NFT6 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 18 KDa |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human DBF4B/ASKL1 |
| Epitope Specificity | 41-140/615 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Nucleus. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction. |
| SIMILARITY | Contains 1 BRCT domain. Contains 1 DBF4-type zinc finger. |
| SUBUNIT | Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4/DBF4A or DBF4B. Such complexes are stable upon replication stress. |
| Post-translational modifications | Phosphorylated. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | DBF4B is a regulatory subunit for CDC7, a serine-threonine kinase which links cell cycle regulation to genome duplication. The complex CDC7-DBF4B selectively phosphorylates the MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. DBF4B localizes to the nucleus and its expression is cell cycle-regulated. |
| Gene ID | 80174 |
|---|---|
| Other Names | Protein DBF4 homolog B, Activator of S phase kinase-like protein 1, ASK-like protein 1, Chiffon homolog B, Dbf4-related factor 1, DBF4B, ASKL1, DRF1 |
| Target/Specificity | Widely expressed. Highly expressed in testis. |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Format | 0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce |
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃. |
| Name | DBF4B |
|---|---|
| Synonyms | ASKL1, DRF1 |
| Function | Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. |
| Cellular Location | Nucleus. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction |
| Tissue Location | Widely expressed. Highly expressed in testis. |
Research Areas
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
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$ 385.00
Cat# AP58303
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