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BRD1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - BRD1 Polyclonal Antibody AP59001
    Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-BRD1 Polyclonal Antibody, Unconjugated(bs-8426R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • IHC-P - BRD1 Polyclonal Antibody AP59001
    Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BRD1) Polyclonal Antibody, Unconjugated (bs-8426R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • IHC-P - BRD1 Polyclonal Antibody AP59001
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-BRD1 Polyclonal Antibody, Unconjugated(bs-8426R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • WB - BRD1 Polyclonal Antibody AP59001
    Sample:
    Testis (Mouse) Lysate at 40 ug
    Primary: Anti- BRD1 (bs-8426R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 119 kD
    Observed band size: 140 kD
    detail
  • WB - BRD1 Polyclonal Antibody AP59001
    Sample:
    Testis (Mouse) Lysate at 40 ug
    Primary: Anti- BRD1 (bs-8426R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size:119 kD
    Observed band size: 150 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession O95696
Reactivity Rat, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 119520 Da
Additional Information
Gene ID 23774
Other Names Bromodomain-containing protein 1, BR140-like protein, Bromodomain and PHD finger-containing protein 2, BRD1 {ECO:0000303|PubMed:16387653, ECO:0000312|HGNC:HGNC:1102}
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name BRD1 {ECO:0000303|PubMed:16387653, ECO:0000312|HGNC:HGNC:1102}
Function Scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, that acts as a regulator of hematopoiesis (PubMed:16387653, PubMed:21753189, PubMed:21880731). Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac), thereby promoting erythroid differentiation (PubMed:21753189).
Cellular Location Nucleus. Chromosome Note=Localizes to transcription start sites
Tissue Location Highly expressed in testis.
Citations (0)
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$ 350.00
Cat# AP59001
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