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TBATA Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TBATA Polyclonal Antibody AP59359
    Sample: A549 Cell (Human) Lysate at 40 ug
    Primary: Anti-C10orf27 (bs-9720R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 39 kD
    Observed band size: 42 kD
    detail
  • IHC-P - TBATA Polyclonal Antibody AP59359
    Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C10orf27) Polyclonal Antibody, Unconjugated (bs-9720R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, E
Primary Accession Q96M53
Reactivity Rat, Pig, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 39 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human C10orf27/SPATIAL
Epitope Specificity 31-130/351
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Cytoplasm,
SIMILARITY Belongs to the TBATA family.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions C10orf27, also known as spatial, is a 351 amino acid cytoplasmic protein belonging to the spatial family. C10orf27 is suggested to play a role in spermatid differentiation. Existing as two alternatively spliced isoforms, C10orf27 is widely expressed in multiple tissues, including brain, thymus and testis. C10orf27 may be associated with multiple sclerosis (MS) susceptibility and pathogenesis. MS is an inflammatory disease that causes gradual destruction of myelin in the central nervous system. C10orf27 is encoded by a gene located on human chromosome 10, which contains over 800 genes and 135 million nucleotides, making up nearly 4.5% of the human genome. PTEN is an important tumor suppressor gene located on chromosome 10 and, when defective, causes a genetic predisposition to cancer development known as Cowden syndrome.
Additional Information
Gene ID 219793
Other Names Protein TBATA, Protein SPATIAL, Stromal protein associated with thymii and lymph node homolog, Thymus, brain and testes-associated protein, TBATA, C10orf27, SPATIAL
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name TBATA
Synonyms C10orf27, SPATIAL
Function May play a role in spermatid differentiation. Modulates thymic stromal cell proliferation and thymus function.
Cellular Location Cytoplasm, cytosol.
Research Areas
Citations (0)
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$ 385.00
Cat# AP59359
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