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EGFR5 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - EGFR5 Polyclonal Antibody AP59390
    Blank control(blue):293T (fixed with 2% paraformaldehyde (10 min)).
    Primary Antibody:Rabbit Anti-EGFR5 antibody(bs-9813R), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
    Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
    detail
  • WB - EGFR5 Polyclonal Antibody AP59390
    Sample:
    B16 Cell (Mouse) Lysate at 40 ug
    DU145 Cell (Human) Lysate at 40 ug
    Primary: Anti-EGFR5 (bs-9813R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 49 kD
    Observed band size: 53 kD
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q86T13
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 51636 Da
Additional Information
Gene ID 161198
Other Names C-type lectin domain family 14 member A, Epidermal growth factor receptor 5, EGFR-5, CLEC14A, C14orf27, EGFR5
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1 µg/Test,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name CLEC14A
Synonyms C14orf27, EGFR5
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)
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$ 350.00
Cat# AP59390
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