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C2orf43 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - C2orf43 Polyclonal Antibody AP59393
    Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C2orf43) Polyclonal Antibody, Unconjugated (bs-9816R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • WB - C2orf43 Polyclonal Antibody AP59393
    Sample:
    Raw264.7(Mouse) Cell Lysate at 30 ug
    Primary: Anti- C2orf43 (bs-9816R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 37 kD
    Observed band size: 35 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, IEM
Primary Accession Q9H6V9
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 37319 Da
Additional Information
Gene ID 60526
Other Names Lipid droplet-associated hydrolase {ECO:0000312|HGNC:HGNC:26145}, 3.1.1.-, Lipid droplet-associated serine hydrolase, hLDAH, LDAH {ECO:0000303|PubMed:24357060, ECO:0000312|HGNC:HGNC:26145}
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,IEM=1:20-200,IGS=1:20-200,GICA=1:20-200,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name LDAH {ECO:0000303|PubMed:24357060, ECO:0000312|HGNC:HGNC:26145}
Function Probable serine lipid hydrolase associated with lipid droplets. Appears to lack cholesterol esterase activity. Appears to lack triglyceride lipase activity. Highly expressed in macrophage-rich areas in atherosclerotic lesions, suggesting that it could promote cholesterol ester turnover in macrophages.
Cellular Location Lipid droplet. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q8BVA5}. Note=Localizes to the endoplasmic reticulum in absence of lipid droplets and translocates to lipid droplets upon lipid storage induction {ECO:0000250|UniProtKB:Q8BVA5}
Tissue Location Present in macrophage-rich areas in atherosclerotic lesionsv(at protein level).
Research Areas
Citations (0)
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$ 350.00
Cat# AP59393
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