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Anti-p47 phox (pS370) Antibody

Rabbit polyclonal antibody to p47 phox (pS370)

     
  • WB - Anti-p47 phox (pS370) Antibody AP59911
    Western blot analysis of p47 phox (pS370) expression in HEK293T (A), mouse lung (B), mouse liver (C), rat lung (D), rat liver (E) whole cell lysates.
    detail
  • IF - Anti-p47 phox (pS370) Antibody AP59911
    Immunofluorescent analysis of p47 phox (pS370) staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
  • E - Anti-p47 phox (pS370) Antibody AP59911
    Direct ELISA antibody dose-response curve using Anti-p47 phox (pS370) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP, IF/IC
Primary Accession P14598
Other Accession Q09014
Reactivity Human, Mouse, Rat, Rabbit
Host Rabbit
Clonality Polyclonal
Calculated MW 44682 Da
Additional Information
Gene ID 653361
Other Names NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox
Target/Specificity Recognizes endogenous levels of p47 phox (pS370) protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
E~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name NCF1
Synonyms NOXO2, SH3PXD1A
Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Cellular Location Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Detected in peripheral blood monocytes and neutrophils (at protein level).
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p47 phox. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP59911
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