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Anti-CDK8 Antibody
Rabbit polyclonal antibody to CDK8
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC |
|---|---|
| Primary Accession | P49336 |
| Other Accession | Q8R3L8 |
| Reactivity | Human, Mouse, Rat, Zebrafish |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 53284 Da |
| Gene ID | 1024 |
|---|---|
| Other Names | Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35 |
| Target/Specificity | Recognizes endogenous levels of CDK8 protein. |
| Dilution | WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200) IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200) |
| Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
| Name | CDK8 |
|---|---|
| Function | Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C- terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. |
| Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDK8. The exact sequence is proprietary.
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