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Anti-NET (pS357) Antibody

Rabbit polyclonal antibody to NET (pS357)

     
  • WB - Anti-NET (pS357) Antibody AP60002
    Western blot analysis of NET (pS357) expression in zebrafish (A) whole cell lysates.
    detail
  • IF - Anti-NET (pS357) Antibody AP60002
    Immunofluorescent analysis of NET (pS357) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP, IF/IC
Primary Accession P41970
Other Accession P41971
Reactivity Human, Mouse, Rat, Zebrafish, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 44240 Da
Additional Information
Gene ID 2004
Other Names NET; SAP2; ETS domain-containing protein Elk-3; ETS-related protein ERP; ETS-related protein NET; Serum response factor accessory protein 2; SAP-2; SRF accessory protein 2
Target/Specificity Recognizes endogenous levels of NET (pS357) protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ELK3
Synonyms NET, SAP2
Function May be a negative regulator of transcription, but can activate transcription when coexpressed with Ras, Src or Mos. Forms a ternary complex with the serum response factor and the ETS and SRF motifs of the Fos serum response element.
Cellular Location Nucleus.
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NET (pS357). The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60002
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