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Anti-AP2 gamma Antibody

Rabbit polyclonal antibody to AP2 gamma

     
  • WB - Anti-AP2 gamma Antibody AP60060
    Western blot analysis of AP2 gamma expression in HEK293T (A), Hela (B), H1688 (C), rat kidney (D), rat lung (E) whole cell lysates.
    detail
  • IH - Anti-AP2 gamma Antibody AP60060
    Immunohistochemical analysis of AP2 gamma staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q92754
Other Accession Q61312
Reactivity Human, Mouse, Rat, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW 49177 Da
Additional Information
Gene ID 7022
Other Names Transcription factor AP-2 gamma; AP2-gamma; Activating enhancer-binding protein 2 gamma; Transcription factor ERF-1
Target/Specificity Recognizes endogenous levels of AP2 gamma protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name TFAP2C
Function Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AP2 gamma. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60060
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