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Anti-Alpha-2A Adrenergic Receptor Antibody

Rabbit polyclonal antibody to Alpha-2A Adrenergic Receptor

     
  • WB - Anti-Alpha-2A Adrenergic Receptor Antibody AP60214
    Western blot analysis of Alpha-2A Adrenergic Receptor expression in Hela (A), A2780 (B) whole cell lysates.
    detail
  • IF - Anti-Alpha-2A Adrenergic Receptor Antibody AP60214
    Immunofluorescent analysis of Alpha-2A Adrenergic Receptor staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP, IF/IC
Primary Accession P08913
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 50647 Da
Additional Information
Gene ID 150
Other Names ADRA2R; ADRAR; Alpha-2A adrenergic receptor; Alpha-2 adrenergic receptor subtype C10; Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2AAR
Target/Specificity Recognizes endogenous levels of Alpha-2A Adrenergic Receptor protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ADRA2A (HGNC:281)
Synonyms ADRA2R, ADRAR
Function Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p- octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.
Cellular Location Cell membrane; Multi-pass membrane protein
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Alpha-2A Adrenergic Receptor. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60214
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