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Anti-IRX3 Antibody

Rabbit polyclonal antibody to IRX3

     
  • WB - Anti-IRX3 Antibody AP60328
    Western blot analysis of IRX3 expression in Hela (A), A549 (B) whole cell lysates.
    detail
  • IH - Anti-IRX3 Antibody AP60328
    Immunohistochemical analysis of IRX3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P78415
Other Accession P81067
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 52119 Da
Additional Information
Gene ID 79191
Other Names IRXB1; Iroquois-class homeodomain protein IRX-3; Homeodomain protein IRXB1; Iroquois homeobox protein 3
Target/Specificity Recognizes endogenous levels of IRX3 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name IRX3
Synonyms IRXB1
Function Transcription factor involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells. Acts as a regulator of energy metabolism.
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IRX3. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60328
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