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Anti-PTTG1IP Antibody

Rabbit polyclonal antibody to PTTG1IP

     
  • WB - Anti-PTTG1IP Antibody AP60377
    Western blot analysis of PTTG1IP expression in mouse kidney (A) whole cell lysates.
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  • IH - Anti-PTTG1IP Antibody AP60377
    Immunohistochemical analysis of PTTG1IP staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-PTTG1IP Antibody AP60377
    Immunofluorescent analysis of PTTG1IP staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession P53801
Other Accession Q8R143
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 20324 Da
Additional Information
Gene ID 754
Other Names C21orf1; C21orf3; Pituitary tumor-transforming gene 1 protein-interacting protein; Pituitary tumor-transforming gene protein-binding factor; PBF; PTTG-binding factor
Target/Specificity Recognizes endogenous levels of PTTG1IP protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name PTTG1IP
Synonyms C21orf1, C21orf3
Function May facilitate PTTG1 nuclear translocation.
Cellular Location Membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus Note=According to PubMed:10781616, it is found in the cytoplasm and the nucleus
Tissue Location Ubiquitous.
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PTTG1IP. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60377
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