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Anti-Tachykinin Receptor 1 Antibody

Rabbit polyclonal antibody to Tachykinin Receptor 1

     
  • WB - Anti-Tachykinin Receptor 1 Antibody AP60400
    Western blot analysis of Tachykinin Receptor 1 expression in HEK293T (A), A2780 (B), mouse spleen (C), mouse kidney (D), rat spleen (E), rat kidney (F) whole cell lysates.
    detail
  • IF - Anti-Tachykinin Receptor 1 Antibody AP60400
    Immunofluorescent analysis of Tachykinin Receptor 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession P25103
Other Accession P30548
Reactivity Human, Mouse, Rat, Chicken, Drosophila
Host Rabbit
Clonality Polyclonal
Calculated MW 46251 Da
Additional Information
Gene ID 6869
Other Names NK1R; TAC1R; Substance-P receptor; SPR; NK-1 receptor; NK-1R; Tachykinin receptor 1
Target/Specificity Recognizes endogenous levels of Tachykinin Receptor 1 protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name TACR1
Synonyms NK1R, TAC1R
Function This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K.
Cellular Location Cell membrane; Multi-pass membrane protein.
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Tachykinin Receptor 1. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60400
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