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Anti-Delta Opioid Receptor (pS363) Antibody

Rabbit polyclonal antibody to Delta Opioid Receptor (pS363)

     
  • WB - Anti-Delta Opioid Receptor (pS363) Antibody AP60495
    Western blot analysis of Delta Opioid Receptor (pS363) expression in A2780 (A), U87MG (B), mouse brain (C), rat muscle (D) whole cell lysates.
    detail
  • IH - Anti-Delta Opioid Receptor (pS363) Antibody AP60495
    Immunohistochemical analysis of Delta Opioid Receptor (pS363) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-Delta Opioid Receptor (pS363) Antibody AP60495
    Immunofluorescent analysis of Delta Opioid Receptor (pS363) staining in SKNSH cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession P41143
Other Accession P32300
Reactivity Human, Mouse, Rat, Pig
Host Rabbit
Clonality Polyclonal
Calculated MW 40369 Da
Additional Information
Gene ID 4985
Other Names OPRD; Delta-type opioid receptor; D-OR-1; DOR-1
Target/Specificity Recognizes endogenous levels of Delta Opioid Receptor (pS363) protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name OPRD1
Synonyms OPRD
Function G-protein coupled receptor that functions as a receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes.
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Delta Opioid Receptor (pS363). The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60495
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