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Anti-CADM2 Antibody

Rabbit polyclonal antibody to CADM2

     
  • WB - Anti-CADM2 Antibody AP60741
    Western blot analysis of CADM2 expression in HEK293T (A), Hela (B), PC3 (C), rat liver (D) whole cell lysates.
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  • IH - Anti-CADM2 Antibody AP60741
    Immunohistochemical analysis of CADM2 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-CADM2 Antibody AP60741
    Immunofluorescent analysis of CADM2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession Q8N3J6
Other Accession Q8BLQ9
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 47554 Da
Additional Information
Gene ID 253559
Other Names IGSF4D; NECL3; Cell adhesion molecule 2; Immunoglobulin superfamily member 4D; IgSF4D; Nectin-like protein 3; NECL-3; Synaptic cell adhesion molecule 2; SynCAM 2
Target/Specificity Recognizes endogenous levels of CADM2 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CADM2
Synonyms IGSF4D, NECL3
Function Adhesion molecule that engages in homo- and heterophilic interactions with the other nectin-like family members, leading to cell aggregation. Important for synapse organization, providing regulated trans-synaptic adhesion. Preferentially binds to oligodendrocytes.
Cellular Location Cell membrane; Single-pass type I membrane protein Synapse. Cell projection, axon. Note=Found in the axoplasm of myelinated axons
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CADM2. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60741
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