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Anti-DIDO1 Antibody

Rabbit polyclonal antibody to DIDO1

     
  • WB - Anti-DIDO1 Antibody AP60746
    Western blot analysis of DIDO1 expression in Hela (A), A2780 (B) whole cell lysates.
    detail
  • IH - Anti-DIDO1 Antibody AP60746
    Immunohistochemical analysis of DIDO1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q9BTC0
Reactivity Human, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW 243873 Da
Additional Information
Gene ID 11083
Other Names C20orf158; DATF1; KIAA0333; Death-inducer obliterator 1; DIO-1; hDido1; Death-associated transcription factor 1; DATF-1
Target/Specificity Recognizes endogenous levels of DIDO1 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name DIDO1
Synonyms C20orf158, DATF1, KIAA0333
Function Putative transcription factor, weakly pro-apoptotic when overexpressed (By similarity). Tumor suppressor. Required for early embryonic stem cell development.
Cellular Location Cytoplasm. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00651}. Cytoplasm, cytoskeleton, spindle. Note=Translocates to the nucleus after pro-apoptotic stimuli (By similarity). Translocates to the mitotic spindle upon loss of interaction with H3K4me3 during early mitosis.
Tissue Location Ubiquitous.
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DIDO1. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60746
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