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Anti-ZP1 Antibody

Rabbit polyclonal antibody to ZP1

     
  • WB - Anti-ZP1 Antibody AP60788
    Western blot analysis of ZP1 expression in mouse kidney (A), rat spleen (B) whole cell lysates.
    detail
  • IH - Anti-ZP1 Antibody AP60788
    Immunohistochemical analysis of ZP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P60852
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 70049 Da
Additional Information
Gene ID 22917
Other Names Zona pellucida sperm-binding protein 1; Zona pellucida glycoprotein 1; Zp-1
Target/Specificity Recognizes endogenous levels of ZP1 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ZP1
Function Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.
Cellular Location [Processed zona pellucida sperm-binding protein 1]: Zona pellucida
Tissue Location Expressed in oocytes (at protein level).
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ZP1. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60788
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