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Anti-DCLK2 Antibody

Rabbit polyclonal antibody to DCLK2

     
  • WB - Anti-DCLK2 Antibody AP60851
    Western blot analysis of DCLK2 expression in H1792 (A), mouse testis (B), mouse heart (C) whole cell lysates.
    detail
  • IF - Anti-DCLK2 Antibody AP60851
    Immunofluorescent analysis of DCLK2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession Q8N568
Other Accession Q6PGN3
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 83606 Da
Additional Information
Gene ID 166614
Other Names DCAMKL2; DCDC3B; DCK2; Serine/threonine-protein kinase DCLK2; CaMK-like CREB regulatory kinase 2; CL2; CLICK-II; CLICK2; Doublecortin domain-containing protein 3B; Doublecortin-like and CAM kinase-like 2; Doublecortin-like kinase 2
Target/Specificity Recognizes endogenous levels of DCLK2 protein.
Dilution WB~~WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)
IF~~WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name DCLK2
Synonyms DCAMKL2, DCDC3B, DCK2
Function Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Note=Colocalizes with microtubules.
Tissue Location Expressed in the brain, heart and eyes.
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DCLK2. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60851
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