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Anti-p46 Antibody

Rabbit polyclonal antibody to p46

     
  • WB - Anti-p46 Antibody AP60976
    Western blot analysis of p46 expression in PC12 (A), CT26 (B), Hela (C), MCF7 (D) whole cell lysates.
    detail
  • IH - Anti-p46 Antibody AP60976
    Immunohistochemical analysis of p46 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q9BY77
Other Accession Q8BG81
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 46089 Da
Additional Information
Gene ID 84271
Other Names KIAA1649; PDIP46; Polymerase delta-interacting protein 3; 46 kDa DNA polymerase delta interaction protein; p46; S6K1 Aly/REF-like target; SKAR
Target/Specificity Recognizes endogenous levels of p46 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/50 - 1/100)
IH~~WB (1/500 - 1/1000), IH (1/50 - 1/100)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name POLDIP3
Synonyms KIAA1649, PDIP46
Function Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Involved in nuclear mRNA export; probably mediated by association with the TREX complex.
Cellular Location Nucleus. Nucleus speckle. Cytoplasm. Note=Nucleocytoplasmic shuttling protein
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p46. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60976
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