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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-CRK (pY221) Antibody   

Anti-CRK (pY221) Antibody

Rabbit polyclonal antibody to CRK (pY221)

     
  • WB - Anti-CRK (pY221) Antibody AP61062
    Western blot analysis of CRK (pY221) expression in Hela (A), THP1 (B), mouse liver (C), rat liver (D) whole cell lysates.
    detail
  • IF - Anti-CRK (pY221) Antibody AP61062
    Immunofluorescent analysis of CRK (pY221) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession P46108
Other Accession Q64010
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 33831 Da
Additional Information
Gene ID 1398
Other Names Adapter molecule crk; Proto-oncogene c-Crk; p38
Target/Specificity Recognizes endogenous levels of CRK (pY221) protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CRK
Function Involved in cell branching and adhesion mediated by BCAR1- CRK-RAPGEF1 signaling and activation of RAP1.
Cellular Location Cytoplasm. Cell membrane. Note=Translocated to the plasma membrane upon cell adhesion.
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CRK. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61062
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