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Anti-C/EBP epsilon (pT74) Antibody

Rabbit polyclonal antibody to C/EBP epsilon (pT74)

     
  • WB - Anti-C/EBP epsilon (pT74) Antibody AP61071
    Western blot analysis of C/EBP epsilon (pT74) expression in Hela (A), mouse liver (B), Raw264.7 (C), rat liver (D) whole cell lysates.
    detail
  • IH - Anti-C/EBP epsilon (pT74) Antibody AP61071
    Immunohistochemical analysis of C/EBP epsilon (pT74) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-C/EBP epsilon (pT74) Antibody AP61071
    Immunofluorescent analysis of C/EBP epsilon (pT74) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession Q15744
Other Accession Q6PZD9
Reactivity Human, Mouse, Rat, SARS
Host Rabbit
Clonality Polyclonal
Calculated MW 30603 Da
Additional Information
Gene ID 1053
Other Names CCAAT/enhancer-binding protein epsilon; C/EBP epsilon
Target/Specificity Recognizes endogenous levels of C/EBP epsilon (pT74) protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500)
IH~~WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CEBPE
Function Transcriptional activator (PubMed:26019275). C/EBP are DNA- binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Required for the promyelocyte-myelocyte transition in myeloid differentiation (PubMed:10359588).
Cellular Location Nucleus
Tissue Location Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human C/EBP epsilon. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61071
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