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Anti-Claudin 4 (pY208) Antibody

Rabbit polyclonal antibody to Claudin 4 (pY208)

     
  • WB - Anti-Claudin 4 (pY208) Antibody AP61098
    Western blot analysis of Claudin 4 (pY208) expression in mouse embryo (A), rat ovary (B), Panc1 (C), HCT116 (D) whole cell lysates.
    detail
  • IH - Anti-Claudin 4 (pY208) Antibody AP61098
    Immunohistochemical analysis of Claudin 4 (pY208) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • E - Anti-Claudin 4 (pY208) Antibody AP61098
    Direct ELISA antibody dose-response curve using Anti-Claudin 4 (pY208) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession O14493
Other Accession O35054
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 22077 Da
Additional Information
Gene ID 1364
Other Names CPER; CPETR1; WBSCR8; Claudin-4; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein
Target/Specificity Recognizes endogenous levels of Claudin 4 (pY208) protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/50 - 1/100)
IH~~WB (1/500 - 1/1000), IH (1/50 - 1/100)
E~~WB (1/500 - 1/1000), IH (1/50 - 1/100)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CLDN4
Function Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Cellular Location Cell junction, tight junction {ECO:0000250|UniProtKB:O35054}. Cell membrane; Multi-pass membrane protein. Note=CLDN4 is required for tight junction localization in the kidney. {ECO:0000250|UniProtKB:O35054}
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Claudin 4. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61098
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