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Anti-CHRM4 Antibody

Rabbit polyclonal antibody to CHRM4

     
  • WB - Anti-CHRM4 Antibody AP61353
    Western blot analysis of CHRM4 expression in C6 (A), SGC7901 (B), U87MG (C), A375 (D) whole cell lysates.
    detail
  • IF - Anti-CHRM4 Antibody AP61353
    Immunofluorescent analysis of CHRM4 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession P08173
Other Accession P32211
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 53049 Da
Additional Information
Gene ID 1132
Other Names Muscarinic acetylcholine receptor M4
Target/Specificity Recognizes endogenous levels of CHRM4 protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CHRM4
Function The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
Cellular Location Cell membrane; Multi-pass membrane protein. Postsynaptic cell membrane; Multi-pass membrane protein
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CHRM4. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61353
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