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Anti-Hairless Antibody

Rabbit polyclonal antibody to Hairless

     
  • WB - Anti-Hairless Antibody AP61384
    Western blot analysis of Hairless expression in rat colon (A), A375 (B), MCF7 (C) whole cell lysates.
    detail
  • IH - Anti-Hairless Antibody AP61384
    Immunohistochemical analysis of Hairless staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-Hairless Antibody AP61384
    Immunofluorescent analysis of Hairless staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC, IHC
Primary Accession O43593
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 127495 Da
Additional Information
Gene ID 55806
Other Names Protein hairless
Target/Specificity Recognizes endogenous levels of Hairless protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
IH~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name HR
Function Histone demethylase that specifically demethylates both mono- and dimethylated 'Lys-9' of histone H3. May act as a transcription regulator controlling hair biology (via targeting of collagens), neural activity, and cell cycle.
Cellular Location Nucleus.
Tissue Location Strongest expression of isoforms 1 and 2 is seen in the small intestine, weaker expression in brain and colon, and trace expression is found in liver, pancreas, spleen, thymus, stomach, salivary gland, appendix and trachea. Isoform 1 is always the most abundant. Isoform 1 is exclusively expressed at low levels in kidney and testis. Isoform 2 is exclusively expressed at high levels in the skin.
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Hairless. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61384
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