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Anti-FOXH1 Antibody

Rabbit polyclonal antibody to FOXH1

     
  • WB - Anti-FOXH1 Antibody AP61441
    Western blot analysis of FOXH1 expression in SGC7901 (A), Myla2059 (B) whole cell lysates.
    detail
  • IF - Anti-FOXH1 Antibody AP61441
    Immunofluorescent analysis of FOXH1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession O75593
Reactivity Human, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 39257 Da
Additional Information
Gene ID 8928
Other Names FAST1; FAST2; Forkhead box protein H1; Forkhead activin signal transducer 1; Fast-1; hFAST-1; Forkhead activin signal transducer 2; Fast-2
Target/Specificity Recognizes endogenous levels of FOXH1 protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
IF~~WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name FOXH1
Synonyms FAST1, FAST2
Function Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).
Cellular Location Nucleus.
Tissue Location Ubiquitous..
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human FOXH1. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61441
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