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Anti-Orexin Receptor 1 Antibody

Rabbit polyclonal antibody to Orexin Receptor 1

     
  • WB - Anti-Orexin Receptor 1 Antibody AP61479
    Western blot analysis of Orexin Receptor 1 expression in U87MG (A), rat brain (B), mouse brain (C) whole cell lysates.
    detail
  • IF - Anti-Orexin Receptor 1 Antibody AP61479
    Immunofluorescent analysis of Orexin Receptor 1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession O43613
Other Accession P58307
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 47536 Da
Additional Information
Gene ID 3061
Other Names Orexin receptor type 1; Ox-1-R; Ox1-R; Ox1R; Hypocretin receptor type 1
Target/Specificity Recognizes endogenous levels of Orexin Receptor 1 protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
IF~~WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name HCRTR1 (HGNC:4848)
Function Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide (PubMed:9491897, PubMed:26950369). Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding (PubMed:9491897, PubMed:26950369).
Cellular Location Cell membrane; Multi-pass membrane protein
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Orexin Receptor 1. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61479
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