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Anti-VEGFA Antibody

Rabbit polyclonal antibody to VEGFA

     
  • WB - Anti-VEGFA Antibody AP61525
    Western blot analysis of VEGFA expression in Hela (A), A549 (B), mouse liver (C), rat liver (D) whole cell lysates.
    detail
  • IH - Anti-VEGFA Antibody AP61525
    Immunohistochemical analysis of VEGFA staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P15692
Other Accession Q00731
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 43597 Da
Additional Information
Gene ID 7422
Other Names VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF
Target/Specificity Recognizes endogenous levels of VEGFA protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200)
IH~~WB (1/500 - 1/1000), IH (1/50 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name VEGFA
Synonyms VEGF
Function [N-VEGF]: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia- mediated cell death (By similarity).
Cellular Location [N-VEGF]: Cytoplasm. Nucleus. Note=Cytoplasmic in normoxic conditions and localizes to the nucleus under hypoxic conditions [Isoform L-VEGF189]: Endoplasmic reticulum. Golgi apparatus. Secreted, extracellular space, extracellular matrix [Isoform VEGF165]: Secreted
Tissue Location Higher expression in pituitary tumors than the pituitary gland. [Isoform VEGF165]: Widely expressed. [Isoform VEGF206]: Not widely expressed.
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human VEGFA. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61525
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