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Anti-Fumarase Antibody

Mouse monoclonal antibody to Fumarase

     
  • WB - Anti-Fumarase Antibody AP61580
    Western blot analysis of Fumarase expression in 293T (A), HepG2 (B), Hela (C), mouse brain (D), rat brain (E) whole cell lysates.
    detail
  • IF - Anti-Fumarase Antibody AP61580
    Immunofluorescent analysis of Fumarase staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession P07954
Other Accession P97807
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Calculated MW 54637 Da
Additional Information
Gene ID 2271
Other Names Fumarate hydratase mitochondrial; Fumarase
Target/Specificity Recognizes endogenous levels of Fumarase protein.
Dilution WB~~WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200)
IF~~WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name FH {ECO:0000303|PubMed:27037871, ECO:0000312|HGNC:HGNC:3700}
Function Catalyzes the reversible stereospecific interconversion of fumarate to L-malate (PubMed:30761759). Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable).
Cellular Location [Isoform Mitochondrial]: Mitochondrion
Tissue Location Expressed in red blood cells; underexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology.
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence of human Fumarase. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61580
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