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Anti-MYOD1 Antibody

Mouse monoclonal antibody to MYOD1

     
  • IH - Anti-MYOD1 Antibody AP61612
    Immunohistochemical analysis of MYOD1 staining in human rhabdomyosarcoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC
Primary Accession P15172
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW 34501 Da
Additional Information
Gene ID 4654
Other Names BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
Target/Specificity Recognizes endogenous levels of MYOD1 protein.
Dilution IH~~WB (1/500 - 1/1000), IH (1/100 - 1/300), IF (1/100 - 1/500)
Format Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name MYOD1
Synonyms BHLHC1, MYF3, MYOD
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within human MYOD1. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP61612
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