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ETV4 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ETV4 Antibody (C-term) AP6642B
    Western blot analysis of ETV4 antibody (C-term) (Cat. #AP6642b) in mouse heart tissue lysates (35ug/lane). ETV4 (arrow) was detected using the purified Pab.
    detail
  • WB - ETV4 Antibody (C-term) AP6642B
    Western blot analysis of ETV4 (arrow) using rabbit polyclonal ETV4 Antibody (C-term) (RB20008). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ETV4 gene (Lane 2) .
    detail
  • IHC-P - ETV4 Antibody (C-term) AP6642B
    Formalin-fixed and paraffin-embedded human brain tissue reacted with ETV4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - ETV4 Antibody (C-term) AP6642B
    Flow cytometric analysis of widr cells using ETV4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P43268
Other Accession P28322
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 53938 Da
Antigen Region 419-447 aa
Additional Information
Gene ID 2118
Other Names ETS translocation variant 4, Adenovirus E1A enhancer-binding protein, E1A-F, Polyomavirus enhancer activator 3 homolog, Protein PEA3, ETV4, E1AF, PEA3
Target/Specificity This ETV4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-447 amino acids from the C-terminal region of human ETV4.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsETV4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ETV4
Synonyms E1AF, PEA3
Function Transcriptional activator (PubMed:19307308, PubMed:31552090). May play a role in keratinocyte differentiation (PubMed:31552090).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00237}.
Tissue Location Expressed in keratinocytes.
Research Areas
Citations ( 0 )

Background

ETV4 is a transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.

References

Wei,Y., J. Biochem. 144 (4), 539-546 (2008)

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$ 365.00
$ 140.00
Cat# AP6642B
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