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> home > Products > Primary Antibodies > Antibody Collections > Ubiquitin E3 Ligases (RING Domain) Antibodies > HIP116A Antibody (Center)
HIP116A Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
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- BACKGROUND
Application 
| FC, WB, E |
|---|---|
| Primary Accession | Q14527 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 113929 Da |
| Antigen Region | 409-437 aa |
| Gene ID | 6596 |
|---|---|
| Other Names | Helicase-like transcription factor, 364-, 632-, DNA-binding protein/plasminogen activator inhibitor 1 regulator, HIP116, RING finger protein 80, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, Sucrose nonfermenting protein 2-like 3, HLTF, HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1 |
| Target/Specificity | This HIP116A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-437 amino acids from the Central region of human HIP116A. |
| Dilution | FC~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HIP116A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HLTF (HGNC:11099) |
|---|---|
| Function | Functions as a DNA-dependent ATPase and E3 ubiquitin-protein ligase involved in chromatin regulation and DNA damage tolerance (DDT) (PubMed:18316726, PubMed:18719106, PubMed:26051180, PubMed:31960921, PubMed:39142279, PubMed:40680746). Catalyzes 'Lys-63'-linked polyubiquitination of monoubiquitinated PCNA at 'Lys-164' in response to genotoxic stress, promoting error-free postreplication repair via template switching (PubMed:18316726, PubMed:18719106). Acts as an epigenetic regulator by promoting recruitment of DNMT1, thereby ensuring DNA methylation inheritance: specifically binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and mediates histone H3 'Lys-23' polyubiquitination (H3K23ub), a docking site for DNMT1, leading to DNMT1 recruitment and replication-coupled DNA methylation maintenance (PubMed:40680746). Catalyzes formation of H3K23ub in two steps: first mediates monoubiquitination together with UBE2E1 and UBE2D2, and then extends ubiquitin chains via 'Lys-63'-linked ubiquitination together with UBE2N and UBE2V2 (PubMed:40680746). Also acts as a chromatin redodeling factor, thereby regulating transcription (PubMed:10391891, PubMed:1994885, PubMed:9126292). Exhibits ATP-dependent double-stranded DNA (dsDNA) translocase activity but lacks classical helicase activity; mediates replication fork reversal by concertedly unwinding and annealing nascent and parental strands, thereby suppressing DNA synthesis and maintaining genomic stability (PubMed:1994885). Resolves G-quadruplex (G4) DNA structures in cooperation with MSH2, limiting replication stress and G4 accumulation across the cell cycle (PubMed:39142279). Contributes to nucleotide excision repair by evicting lesion-containing oligonucleotides using its HIRAN and ATPase domains (PubMed:26051180). Can displace single-stranded DNA from triplex structures through ATP-dependent dsDNA translocation (PubMed:26051180, PubMed:31960921). Also has protein clearing activity at the stalled replication fork, facilitating restart of DNA replication (PubMed:21795603). |
| Cellular Location | Nucleus. Chromosome |
| Tissue Location | Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
HIP116A is a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. This protein contains a RING finger DNA binding motif.
References
Ding,H., et.al., DNA Cell Biol. 15 (6), 429-442 (1996)
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$ 75.00
⚠️ Limit: 5 vials per order for discount.
$ 192.50
⚠️ Limit: 5 vials per order for discount.
Cat# AP6812c
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