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RS18 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - RS18 Antibody (Center) AP6887c
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2OS cells labeling RPS18 with AP6887C at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG (OH191631) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (1186255) at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).
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  • WB - RS18 Antibody (Center) AP6887c
    All lanes : Anti-RS18 Antibody (Center) at 1:1000 dilution Lane 1: U-251 MG whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: THP-1 whole cell lysate Lane 4: Daudi whole cell lysate Lane 5: Mouse brain lysate Lane 6: Mouse spleen lysate Lane 7: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • IHC-P - RS18 Antibody (Center) AP6887c
    Immunohistochemical analysis of AP6887C on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P - RS18 Antibody (Center) AP6887c
    Immunohistochemical analysis of AP6887C on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P-Leica - RS18 Antibody (Center) AP6887c
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using AP6887C performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • FC - RS18 Antibody (Center) AP6887c
    Overlay histogram showing U-2 OS cells stained with AP6887C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6887C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, FC, IF, IHC-P-Leica, E
Primary Accession P62269
Other Accession P62271, P62272, P62270, Q3T0R1
Reactivity Human, Mouse, Rat
Predicted Bovine, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 17719 Da
Antigen Region 62-91 aa
Additional Information
Gene ID 6222
Other Names 40S ribosomal protein S18, Ke-3, Ke3, RPS18, D6S218E
Target/Specificity This RS18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the Central region of human RS18.
Dilution IF~~1:25
WB~~1:1000
IHC-P~~1:100
IHC-P-Leica~~1:500
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRS18 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS18
Synonyms D6S218E
Function Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
citation

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Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm.

References

Yu,Y., Ji,H., et.al., Protein Sci. 14 (6), 1438-1446 (2005)

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$ 350.00
$ 117.00
Cat# AP6887c
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