BRD3 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
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Primary Accession | Q15059 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 79542 Da |
Antigen Region | 672-702 aa |
Gene ID | 8019 |
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Other Names | Bromodomain-containing protein 3, RING3-like protein, BRD3, KIAA0043, RING3L |
Target/Specificity | This BRD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672-702 amino acids from the C-terminal region of human BRD3. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | BRD3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | BRD3 {ECO:0000303|PubMed:18406326, ECO:0000312|HGNC:HGNC:1104} |
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Function | Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures (PubMed:18406326, PubMed:32895492, PubMed:22464331, PubMed:27105114). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed:32895492, PubMed:29567837). In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (PubMed:18406326). Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression (PubMed:32895492). Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity). |
Cellular Location | Nucleus. Chromosome. Note=Detected on chromatin |
Tissue Location | Ubiquitous.. |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.
References
Kaneko, H., et al., J Investig Allergol Clin Immunol 12(2):86-90 (2002).
Thorpe, K.L., et al., Immunogenetics 44(5):391-396 (1996).
Thorpe, K.L., et al., Gene 200 (1-2), 177-183 (1997).
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