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CAT Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | P04040 |
| Other Accession | P04762, O62839, P24270, Q9PT92, P00432 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Zebrafish, Pig, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 59756 Da |
| Antigen Region | 152-180 aa |
| Gene ID | 847 |
|---|---|
| Other Names | Catalase, CAT |
| Target/Specificity | This CAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the Central region of human CAT. |
| Dilution | FC~~1:10~50 IHC-P~~1:50~100 WB~~1:2000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CAT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CAT |
|---|---|
| Function | Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide (PubMed:7882369). Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells (PubMed:7882369). |
| Cellular Location | Peroxisome matrix |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. It promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
References
Oh,J.H., et.al., Mamm. Genome 16 (12), 942-954 (2005)
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Anonymous
2016-08-24 01:15:32
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Species tested
Human,Mouse,Rat
Application tested
WB
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose |
 |
$ 150.00
$ 385.00
Cat# AP8623c
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