GLIS1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | Q8NBF1 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 65976 Da |
Antigen Region | 133-161 aa |
Gene ID | 148979 |
---|---|
Other Names | Zinc finger protein GLIS1, GLI-similar 1, GLIS1 (HGNC:29525) |
Target/Specificity | This GLIS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-161 amino acids from the N-terminal region of human GLIS1. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | GLIS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | GLIS1 (HGNC:29525) |
---|---|
Function | Acts both as a repressor and an activator of transcription (PubMed:21654807). Binds to the consensus sequence 5'-GACCACCCAC-3' (By similarity). By controlling the expression of genes involved in cell differentiation inhibits the lineage commitment of multipotent cells (PubMed:21654807). Prevents, for instance, the differentiation of multipotent mesenchymal cells into adipocyte and osteoblast (By similarity). |
Cellular Location | Nucleus {ECO:0000250|UniProtKB:Q8K1M4}. |
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Provided below are standard protocols that you may find useful for product applications.
Background
GLIS1 is a GLI (MIM 165220)-related Kruppel-like zinc finger protein that functions as an activator and repressor of transcription (Kim et al., 2002 [PubMed 12042312]).
References
Kim,Y.S., et.al., Nucleic Acids Res. 31 (19), 5513-5525 (2003)
Nakanishi,G., et.al., J. Invest. Dermatol. 126 (1), 49-60 (2006)
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