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GFP-Tag Mouse Monoclonal Antibody

Mouse mAb

     
  •  - GFP-Tag Mouse Monoclonal Antibody AP90005
    Western blot analysis of GFP protein expression in (1) 293T cell lysate; (2) 293T cell transfected with GFP protein lysate.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, IP
Primary Accession P42212
Reactivity All Species
Clonality Monoclonal
Other Names GFP ; yfp ; Green fluorescent protein ;
Isotype Mouse IgG1
Host Mouse
Calculated MW 26886 Da
Additional Information
Dilution WB 1:3000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:100
Purification Affinity-chromatography
Immunogen Full-length GFP protein
Description Green fluorescent protein(GFP) has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.
Storage Condition and Buffer Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Protein Information
Name GFP
Function Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Tissue Location Photocytes.
Citations (0)
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$ 350.00
$ 175.00
Cat# AP90005
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Availability: 4 weeks
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