|Application ||WB, FC, IHC-P, E|
|Calculated MW||12155 Da|
|Antigen Region||50-78 aa|
|Other Names||Cytochrome c oxidase subunit 6A1, mitochondrial, Cytochrome c oxidase polypeptide VIa-liver, Cytochrome c oxidase subunit VIA-liver, COX VIa-L, COX6A1, COX6AL|
|Target/Specificity||This COX6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the Central region of human COX6A1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||COX6A1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol- cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.|
|Cellular Location||Mitochondrion inner membrane; Single-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Hochstrasser D.F., et.al., Electrophoresis 13:992-1001(1992).
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