FBXO4 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q9UKT5 |
Other Accession | Q8CHQ0, Q3T0J1 |
Reactivity | Human |
Predicted | Bovine, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 44136 Da |
Antigen Region | 225-251 aa |
Gene ID | 26272 |
---|---|
Other Names | F-box only protein 4, FBXO4, FBX4 |
Target/Specificity | This FBXO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-251 amino acids from the Central region of human FBXO4. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | FBXO4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | FBXO4 |
---|---|
Synonyms | FBX4 |
Function | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:18598945, PubMed:10531035, PubMed:29142209, PubMed:20181953). Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation (PubMed:18598945). Recognizes TERF1 and promotes its ubiquitination together with UBE2D1 (PubMed:16275645, PubMed:20159592). Promotes ubiquitination of FXR1 following phosphorylation of FXR1 by GSK3B, leading to FXR1 degradation by the proteasome (PubMed:29142209). |
Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q8CHQ0}. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
FBXO4 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein belongs to the Fbxs class.
References
Zeng,Z., et.al., Dev. Cell 18 (2), 214-225 (2010)
Barbash,O., et.al., Cancer Cell 14 (1), 68-78 (2008)
Sugiyama,N., et.al., Mol. Cell Proteomics 6 (6), 1103-1109 (2007)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.