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PPP3R2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PPP3R2 Antibody (N-term) AP9384a
    Western blot analysis of PPP3R2 Antibody (N-term) (Cat. #AP9384a) in mouse brain tissue lysates (35ug/lane). PPP3R2 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - PPP3R2 Antibody (N-term) AP9384a
    Formalin-fixed and paraffin-embedded human testis tissue reacted with PPP3R2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - PPP3R2 Antibody (N-term) AP9384a
    PPP3R2 Antibody (N-term) (Cat. #AP9384a) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q96LZ3
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 19533 Da
Antigen Region 3-29 aa
Additional Information
Gene ID 5535
Other Names Calcineurin subunit B type 2, Calcineurin B-like protein, CBLP, Calcineurin BII, CNBII, PPP3R1-like, Protein phosphatase 2B regulatory subunit 2, Protein phosphatase 3 regulatory subunit B beta isoform, PPP3R2, CBLP, PPP3RL
Target/Specificity This PPP3R2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-29 amino acids from the N-terminal region of human PPP3R2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPP3R2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP3R2
Synonyms CBLP, PPP3RL
Function Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Cellular Location Mitochondrion {ECO:0000250|UniProtKB:Q63811}. Note=Localizes in the mitochondria in a SPATA33-dependent manner {ECO:0000250|UniProtKB:Q63811}
Tissue Location Testis-specific..
Research Areas
Citations (0)
citation

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Background

PPP3R2 is regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. This protein confers calcium sensitivity (By similarity).

References

He, Z.H., et al. Eur. J. Appl. Physiol. (2010) In press :
Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957(2009)
Chow, R., et al. Biochem. Biophys. Res. Commun. 363(3):506-512(2007)
Saeki, M., et al. J. Biol. Chem. 282(16):11786-11794(2007)

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$ 182.50
$ 70.00
Cat# AP9384a
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