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RSL1D1 Rabbit pAb

RSL1D1 Rabbit pAb

     
  • IHC-P - RSL1D1 Rabbit pAb AP93974
    Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RSL1D1) Polyclonal Antibody, Unconjugated (AP93974 ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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  • WB - RSL1D1 Rabbit pAb AP93974
    Sample: Lane 1: Heart (Rat) Lysate at 40 ug Lane 2: Heart (Mouse) Lysate at 40 ug Lane 3: Muscle (Rat) Lysate at 40 ug Lane 4: Muscle (Mouse) Lysate at 40 ug Lane 5: Placenta (Rat) Lysate at 40 ug Lane 6: Placenta (Mouse) Lysate at 40 ug Lane 7: Cerebrum (Rat) Lysate at 40 ug Lane 8: Cerebrum (Mouse) Lysate at 40 ug Lane 9: Testis (Rat) Lysate at 40 ug Lane 10: Testis (Mouse) Lysate at 40 ug Lane 11: Uterus (Rat) Lysate at 40 ug Lane 12: Uterus (Mouse) Lysate at 40 ug Primary: Anti-RSL1D1 (AP93974) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 52 kD
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  • IHC - RSL1D1 Rabbit pAb AP93974
    Standard curves generated using the respective human IgG and IgCw-γκ. ELISA data from the right-hand panels were used to plot the log graphs. Human IgG and IgCw-γ with known concentrations were placed in wells coated with goat anti-human IgG/Fc antibody as a capture antibody, and bound Ig molecules were detected with AP-conjugated goat anti-human Cκ antibody F (ab')2 as a detection antibody. Data are presented as mean ± SD, n = 3. Fig. 4. PMID: 30910358.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 55 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from mouse RSL1D1
Epitope Specificity 311-410/452
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Nucleus
SIMILARITY Belongs to the ribosomal protein L1P family. Highly divergent.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions RSL1D1, also known as CATX-11, PBK1, L12 or CSIG, is a 490 amino acid nuclear protein that belongs to the ribosomal protein L1P family. Expressed in placenta, RSL1D1 contains many phosphorylated amino acid residues and is encoded by a gene that maps to human chromosome 16p13.13. Chromosome 16 encodes over 900 genes in approximately 90 million base pairs, makes up nearly 3% of human cellular DNA and is associated with a variety of genetic disorders. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, though through the CREBBP gene which encodes a critical CREB binding protein. Signs of Rubinstein-Taybi include mental retardation and predisposition to tumor growth and white blood cell neoplasias.
Additional Information
Target/Specificity Placenta
Dilution WB=1:500-2000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Citations (0)
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Background

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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$ 385.00
Cat# AP93974
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