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ASH1L Rabbit pAb

ASH1L Rabbit pAb

     
  • IHC-P - ASH1L Rabbit pAb AP94295
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KMT2H/ASH1L Polyclonal Antibody, Unconjugated(AP94295) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF
Primary Accession Q9NR48
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 332790 Da
Additional Information
Gene ID 55870
Other Names Histone-lysine N-methyltransferase ASH1L, 2.1.1.359, 2.1.1.367, ASH1-like protein, huASH1, Absent small and homeotic disks protein 1 homolog, Lysine N-methyltransferase 2H, ASH1L, KIAA1420, KMT2H
Dilution IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name ASH1L
Synonyms KIAA1420, KMT2H
Function Histone methyltransferase specifically trimethylating 'Lys- 36' of histone H3 forming H3K36me3 (PubMed:21239497). Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity). The physiological significance of the H3K9me1 activity is unclear (By similarity).
Cellular Location Nucleus. Cell junction, tight junction. Chromosome. Note=The relevance of tight junction localization is however unclear.
Tissue Location Widely expressed, with highest level in brain, heart and kidney.
Research Areas
Citations (0)
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Background

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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$ 385.00
Cat# AP94295
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